The ZEN module processes up to 8 different channels and, besides the spatial image coordinates, also takes the time lapse informationrelating to your images into consideration. With 3D VisArt you can display multi-dimensional image stacks as 3D volume models. This means that fine structures on the specimen can no longer be picked out. Meaningful results on the colocalization of proteins can be obtained as a scatter plot, image and data table.ĭue to the optical properties of the microscope system, light from the areas above and below the focal plane also enters the image.
ZEN LITE MANUAL SOFTWARE
To do this, the ZEN software makes use of modern methods, such as the automated threshold value method of Costes et al. The Colocalization module can be used to unmix the overlapping signals of different fluorescence channels. This means that important details from the individual images of your microscope specimen are recorded in a single image. The advantage: even images that have been acquired offset from each other can be joined precisely using ZEN and Panorama. The software puts the images together with pixel precision. With Panorama you create high-resolution overview images from individual images. This ZEN module is ideal if your specimens no longer fit into the image field. No calibration procedure is required for this and the focus is recorded automatically for all objectives and contrast techniques. ZEN “learns” the ideal position during your everyday work on the light microscope and is therefore trained continuously with regard to correct focusing. With the Autofocus module for widefield systems you determine the optimal focus position for your specimen in transmitted light, reflected light and reflected light fluorescence. such as time series, Z-stacks, tile/multiposition images and channels, in a clearly visualizable form using experiment blocks. You plan combinations or repeats of your image acquisition tasks. Would you like to plan complex experiments in advance, but retain complete control at all times? From now on ZEN will help you do this! Using the Experiment Designer module you will even be able to create images in several combined image dimensions with just a few clicks of your mouse, and after only a short training period. With the Time Lapse module, damage is no longer caused to specimens as a result of unnecessarily long exposure times – an important requirement for your quantitative analyses of living specimens. ZEN controls the exposure setting for your microscope here with millisecond precision. This allows you to document dynamic processes in your specimens with a compact image sequence. With the ZEN Time Lapse module for widefield systems you control your microscope, camera and imaging software from Carl Zeiss. Tiles & Positions and ZEN align the individual images precisely in relation to one another, providing the optimal basis for further analyses. This allows you to navigate over the specimen like a map and zoom easily into any regions of interest. A large, virtual overview image can be generated in this way from numerous individual images. With the optional ZEN Tiles & Positions module and a motorized stage you scan your specimen automatically, making it possible to analyze large areas. The advantage: you always have maximum depth of field in all regions of your images in transmitted light, reflected light and fluorescence contrast. The ZEN module extracts sharply imaged details from existing images at different focus positions and combines them to create a new image. With the Extended Focus module you generate images with extended depth of field from previously acquired Z-stacks. ZEN can calculate the increments fully automatically, if required, or you can allocate them manually. The image acquisition program controls the Z-drive of your stand in precisely the right increments and synchronizes it with acquisition. ZEN Z Stack module helps you to acquire axial image stacks on your ZEISS microscope with motorized focus drive. This already contains data sets for more than 500 fluorochromes and can be expanded as required. A special feature here is that you can select your dyes from the integrated database. phase contrast, according to your requirements. To do this, with widefield systems you combine any number of fluorescence channels with images in transmitted light, e.g. With ZEN Multi Channel image acquisition module you create images with m ultiple channels. You will find the optimal ZEN module for every task here. Thanks to its modular concept, however, the software guarantees you other advantages too: you can tailor ZEN precisely to your requirements and only need to learn to work with the functions you actually need. Even in the basic version ZEN offers countless functions for day-to-day work with your microscope.